Rat Anti Brdu mAb

General description:

5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light. Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that is selectively incorporated into cell DNA at the S phase of the cell cycle.
Anti-BrdU antibody, Mouse monoclonal (mouse IgG1) is derived from the BU-33 hybridoma produced by the fusion of murine myeloma cells and splenocytes from BALB/c mouse immunized with bromodeoxyuridine (BrdU) conjugated to KLH.5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo.

Target Species:

Chemical

Product Form:

Purified IgG - lyophilized

Reconstitution:

Reconstitute with 0.1 ml distilled water

Preparation:

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution:

Phosphate buffered saline

Preservative Stabilisers:

5% Trehalose

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml

Regulatory:

For research purposes only

Guarantee:

12 months from date of despatch

Acknowledgements:

PrecisionAb is a trademark of Bio-Rad Laboratories

Specificity:

Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed.

Immunogen:

bromodeoxyuridine conjugated to KLH.

Application:

Anti-BrdU antibody has been used in:

• BrdU labeling
• immunohistochemistry
• immunostaining
• BrdU assay

Anti-BrdU antibody, Mouse monoclonal has been used in immunostaining, as a primary antibody:

• to detect 5-fluorouridine-labelled RNA
• to detect 5-bromo-2′-deoxyuridine (BrdU) labelled immunoreactive cells
• against BrdU labelled rat retinal sections

Packaging:

200 μL in glass bottle

Physical form:

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.

Storage and Stability:

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.